Thermo Scientific CryoMed Controlled Rate Freezer
Click here to see used and refurbished Thermo Scientific CryoMed freezer from American Instrument Exchange.
Controlled rate freezers can be used to prepare samples prior to cryogenic storage, ensuring maximum viability. These units are great for a number of reasons, notably that they are low energy and emissions operators: because LN2 storage depends on the cooling power of naturally sourced nitrogen gas, there are no halogenated hydrocarbon refrigerants used and no greenhouse gas concerns.
The Thermo CryoMed model 7452 is a front loading unit with a 34L capacity and a chamber temperature range from 50°C to -180°C. It features an integrated control system (consisting of freezing chamber, microprocessor control and printer – all in one bench top unit), allowing multiple freezing profiles to be tailored to specific protocols. Built-in dual solenoid valves increase LN2 injection volume for precise temperature control and accelerated freezing. This unit is specifically designed air handling system and patented liquid nitrogen injection ring work in tandem for precise temperature control and uniformity while over-temperature protection for heating provides a safe working environment. Standard alarms provide for open thermocouple, heater malfunction, high/low temperature limit, temperature tracking, power failure and completion of run.
Six pre-set profiles are factory programmed while an additional ten user defined profiles can be programmed by the customer. These profiles included:
- Pre-Set Profile #1 – Commonly used for 2.0ml sample size, resulting in a 1°C rate from nucleation to -40°C and a 10°C per minute cooling rate to a -90°C end temperature.
- Pre-Set Profile #2 – Commonly used for very small (96 well) sample size, resulting in a 1°C rate from nucleation to -40°C and a 10°C per minute cooling rate to a -90°C end temperature.
- Pre-Set Profile #3 – Commonly used for straw size samples, resulting in a slow rate to -7°C, a 5 minute hold for manual cell nucleation, a 0.3°C rate to -35°C, and then a 0.1°C per minute cooling rate to a -37°C end temperature.
- Pre-Set Profile #4 – Commonly used for hemopoietic stem cells that are 65-100ml in size, resulting in a 1°C rate from nucleation to -45°C and a 10°C per minute cooling rate to a -90°C end temperature.
- Pre-Set Profile #5 – Commonly used for skin packets, resulting in a 1°C rate from nucleation to -35°C and a 10°C per minute cooling rate to a -90°C end temperature.
- Pre-Set Profile #6 – Commonly used for hemopoietic stem cells that are 65-100ml in size, resulting in a 0.5°C rate from nucleation to -20°C and a 1°C per minute cooling rate to a -80°C end temperature.
If you’re looking for a controlled rate freezer for your lab please give us a call or email today about the Thermo CryoMed 7452. Thanks!
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